DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Bone Marrow, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Chemgenes Corporation DMT-1972
DMT-1972 is a selective 5-HT2A antagonist with an IC50 of 0.34 nM, over 10,000 nM for 5-HT2C. Useful for studying 5-HT2A's role in mental disorders, it's a crystalline solid, soluble in DMSO and DMF, and should be stored at -20°C. Molecular weight: 438.90 g/mol.
Enzo Life Sciences Human Genomic DNA, Female (100µg)
Human Genomic DNA, Female, is comprised of a pool of ≥ 8 normal donors. It is intact DNA extracted from freshly harvested whole blood of healthy females. DNA is treated with DNase free-RNase to remove residual contaminant RNA. Human genomic DNA can be used for a variety of molecular applications including PCR, southern blotting, and DNA library construction. It can also be used as normal reference DNA for comparative genomic hybridizations (CGH). Alternative name: gDNA. Concentration: 150 - 250 µg/mL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA. Formulation: Liquid. Long Term Storage: -20°C.
Zymo Research Corporation ZymoBIOMICS-96 DNA Kit (lysis Matrix Not Included) (2 x 96 preps)
The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies1 of different organisms (e.g. Gram-negative/positive bacteria including endospores2 , fungi, protozoans, algae, etc.) making it ideal for microbial community profiling. Unbiased mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standard.In addition, the ZymoBIOMICS™ 96 DNA Kit is equipped with Zymo Researchs proprietary OneStep™ PCR InhibitorRemoval technology, enabling PCR from the most PCR prohibitive environmental samples rich in humic and fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology with Zymo-Spin™ Technology results in superior yields of ultra-pure DNA ideal for all downstreamapplications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.
Research Products International Corp ZymoPURE Plasmid Miniprep Kit, 400 Preps
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
Bio Rad Laboratories INSTAGENE MATRIX
InstaGene matrix is a specially formulated, 6% weight to volume Chelex resin for the rapid isolation of small amounts of genomic DNA of sufficient purity for PCR. The 20 ml volume is sufficient for 100 samples. (732-6030)
Zymo Research Corporation ZR Plasmid Miniprep™-Classic Kit (100 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin IIN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (400 Preps)
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l).In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Thomas Scientific DirectAmpT Genomic DNA Amplification Kits, Preparation Solution for DirectAmpT Tissue DNA Extraction & Amplification Kit, 100 reactions
Extract genomic DNA in 15 minutes Tissue, plant or seed Quick isolation and amplification for genotyping Highly specific PCR amplification Eliminates complicated purifications, centrifugation and extractions Denville DirectAmp™ Genomic DNA Amplification Kits contain all reagents for rapid extraction and amplification of genomic DNA from a variety of sources. These kits provide a simple and quick extraction method that eliminates protocol steps that call for liquid nitrogen freezing, enzyme digestions, hazardous organic extractions, column purification, centrifugation or precipitation. Each kit includes Denville Hot Start Blue Mastermix, noted for its highly specific amplification of targeted genomic DNA, without adding loading buffer or tracking dye for gel analysis. Although included as a standard kit component, Hot Start Blue Mastermix is also available separately.
Research Products International Corp Quick-DNA/RNA MiniPrep Kit, 50 Preps
The Quick-DNA/RNA Miniprep Kit provides a quick method for the isolation of high quality genomic DNA and total RNA from small amounts of cells and tissue. The kit isolates both genomic DNA and a broad range of RNA species without the use of phenol. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol – no extra steps are required! Both DNA and RNA from up to 5x106 cells can be eluted into volumes as little as 25 µl in less than 15 minutes.
Quick & Easy: Extract DNA and RNA from cells, small amounts of easy-to-lyse tissue, buffy coat, buccal cells, plasma, serum, and other biological liquids. Not compatible with whole blood.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
Norgen Biotek Corp Urine DNA Isolation Micro Kit
This kit provides a fast, reliable and simple procedure for isolating DNA from up to 1.75 mL of urine. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. Multiple samples can be processed in 30 minutes. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more. This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.
Magbio Genomics Inc HighPrep Blood & Tissue DNA Kit (384 preps)
The HighPrep™ Blood & Tissue DNA kit is a magnetic beads based system to process high quality genomic DNA from 50-250 µL of fresh or frozen whole blood, buffy coat (containing anticoagulants such as Citrate, EDTA and Heparin), lysate of tissues, mouse tails, cultured cells, saliva, or buccal swabs.The HighPrep Blood & Tissue DNA kit is highly geared for automation where high quality genomic DNA is reproducible in a high throughput environment. The kit is adaptable to commercially available liquid handling workstations.
Biochain Institute Inc Genomic DNA - Arrhythmia, infarct: Interventricular Septum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - hypertension: Heart Atrium, left, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Control Genomic DNA - Porcine Female, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.