DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Small Intestine, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Carnabio Usa Inc AURA(AURKA)/5UG/FULL-LENGTH
AurA(AURKA), Full-length, Wild type, Amino Acid 1-403, NP_940835.1, Expressed in Insect (sf21); 5microg
Enzo Life Sciences AMPIPROBE Bacterial Vaginosis Extraction Controls (500µl)
The AMPIPROBE Candida Extraction Controls are designed to be used during the genomic DNA extraction of Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp., Megasphaera spp., and BVAB2. All performance specifications must be validated by end-user and will vary based on chosen extraction methods. Eluent from extraction can be used as extraction control for AMPIPROBE Bacterial Vaginosis Assay kit (ENZ-GEN205). Plasmids in Extraction Controls are present at higher concentrations compared to assay controls included in the qPCR kit. Extra care should be taken to avoid contamination. Applications: qPCR. Species reactivity: BVAB-2, Megasphaera-1, Lactobacillus spp., Gardnerella vaginalis, Atopobium vaginae.
Bulldog Bio Inc FastGene Scriptase II ReadyMix (100 rxns), OdT & Random Hex-primers
How would you construct the ideal reverse transcriptase? It would have to make very long cDNAs at high yields. It would have to be able to work from very small amounts of input RNA. And of course, it would have to work fast. FastGene Scriptase II checks all three boxes, and it does it at a reasonable price. Here’s how. First, mutations are inserted into the RNase H domain of native MuLV‘s reverse transcriptase delivering a higher yield of full-length cDNAs. Next, changes are made to increase the enzymes natural thermal stability providing a more robust enzyme that doesn’t lose processing power, even at elevated temperatures. Finally, the reaction buffer composition is optimized for high speed production of cDNAs from incredibly low levels of input RNA. This unique combination of size, speed, and production makes FastGene Scriptase II the optimal choice for even the most complex applications requiring cDNAs, such as RT-qPCR and NGS.
Research Products International Corp Quick-DNA/RNA MiniPrep Plus Kit, 50 Preps
The Quick-DNA/RNA MiniPrep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a unique preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from any biological sample including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality genomic DNA and total RNA that is ready for any downstream application including reverse transcription, microarray, sequencing, etc.
Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Liver, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Alkali Scientific Radiant™ Extract & Amplify Tissue PCR Kit 80 Reactions 2 x 1mL 2X HS Taq Red, 1 x 1.6mL 5X Extract Buffer A, 1 x .08mL 10X Extract Buffer B
Radiant™ Extract and Amplify Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tail, ear punch and other mammalian tissues, buccal swabs, hair shafts, and saliva. SPEED UP your GENOTYPING NO OVERNIGHT lysing Protocol: Extraction Solutions, included: 15 minutes then PCR as directed with supplied Radiant™ RED 2X mix During the quick 5 minute extraction process, DNA is released from the starting material by mixing the sample with ASI's proprietary Extraction Solution. This is followed by a 10 minute incubation at 95 degree to help inactivate proteins and genomic DNA. NO OVERNIGHT EXTRACTION. There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA.
Biochain Institute Inc Genomic DNA - Parkinson's Disease: Brain: Corpus Callosum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation Quick-DNA Tissue/Insect Microprep Kit (50 Preps)
The procedure is easy and can be completed in as little as 15 minutes: samples are added directly to a ZR BashingBead™ Lysis Tube (2.0 mm), then rapidly and efficiently lysed by bead, without using organic denaturants or proteinases. The DNA is isolated and purified using our Zymo-Spin™ Technology and is ideal for downstream molecular-based applications including PCR, array, genotyping, etc. The procedure is compatible with mammalian tissues, whole blood,and cultured cells.
Biochain Institute Inc xTractPure FFPE RNA Extraction Kit, 1 kit/pk
xTractPure FFPE RNA Extraction Kit, 1 kit/pk
Omega Biotek Inc Mag-Bind SeqDTR, Magnetic Bead-based purification system, 500 mL, Manual or automated, 96-well, 384-well, Over 800 bps
The Omega Bio-tek Mag-Bind SeqDTR is a magnetic bead-based purification system designed for the efficient and reliable removal of unincorporated dye terminators from sequencing reactions. This product employs proprietary chemistry and utilizes the unique properties of paramagnetic beads, enhancing sample purity and quality in sequencing applications. It is suitable for both manual and automated workflows, allowing users to achieve optimal performance with ease.
- Application: Removal of unincorporated dye terminators from sequencing reactions
- Technology: Magnetic bead based
- Kit volume: 500 mL
- Automation compatibility: Manual or automated
- Average read lengths: Over 800 bps (Min Phred 20)
- Reduce reagent usage: 1/16 or 1/32 compared to sephadex cleanups
- Contaminants removed: Nucleotides, primers, salts, dye terminators
- Storage conditions: 2-8 °C
- Processing time: Less than 25 minutes for 4 plates
Bioworld Blood Genomic DNA Isolation Midi, 12 Prep(s)
For the rapid preparation of high quality DNA from whole blood, plasma and serum Input volumes of 500 µL to 2 mL No phenol required for DNA isolation Extract DNA from whole blood, plasma and serum Fast and convenient spin column protocol FEATURES AND BENEFITSNo Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes. Isolate genomic DNA from various inputs - Isolate DNA from various blood inputs including plasma, whole blood and serum Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis. High quality DNA with no RNA contamination - No contamination or degradation of genomic DNA isolated with the Blood Genomic DNA Isolation Midi Kit is observed.
Research Products International Corp Zymo ZymoPURE-Express Plasmid MidiPrep Kit, 25 Preps
The ZymoPURE - Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the pelleting and resuspension steps. Just add the ZP Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin V-P microcentrifuge column. The ZymoPURE - Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. The innovative binding chemistry (patent pending) enables highly concentrated DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The eluted plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
New England Biolabs, Inc. NEBNext dA-Tailing Module – 20 reactions
This module is part of the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 µg of input DNA vs. 500 pg - 1 µg required for the Ultra II DNA workflow.